Lecture by Prof. Daniel Häussinger from University of Basel, Switzerland, entitled „Lanthanide Chelating Tags in Structural Biology“ from the CAPSID Seminar Speaker Series, will take place on 11 March 2021 at 2 p.m. via online broadcasting via the ZOOM application.
RESEARCH FOCUS:
Nuclear magnetic resonance spectroscopy provides an ideal toolbox for elucidating structure, dynamics, and interactions of biomacromolecules in solution, since the offered possibility to tune and adjust the experimental conditions, i.e. buffer components, salt concentration, additives, temperature, and pH, yields realistic and atomic-resolution structures of biomacromolecules under physiological conditions. In order to study protein-protein or protein-ligand interactions as well as the positioning and conformation of flexible domains within proteins in solution, long-range structural restraints are urgently needed. Paramagnetic nuclear magnetic resonance spectroscopy, more specifically pseudo-contact shifts and residual dipolar couplings generated by lanthanide chelating tags, can deliver such long-range restraints and thereby render amenable the structural analysis of large protein complexes as well as protein-ligand binding in solution.
RESEARCH HIGHLIGHT:
“Design of next-generation lanthanide chelating tags”
In order to generate strongly paramagnetic lanthanide chelating tags that are sufficiently immobilized on the surface of the protein and generate thereby large paramagnetic effects, i.e. large pseudo-contact shifts and residual dipolar couplings, we synthesize sterically overcrowded DOTA-derived lanthanide chelating tags with different linker systems. More specifically, the introduction of sterically demanding substituents, e.g. isopropyl groups, as well as the introduction of novel, reduction-stable linker moieties, e.g. pyridinethiazole derivatives, proved to be highly beneficial in order to generate lanthanide chelating tags with optimal properties and general applicability.
More information about lecture and registration on the project web site: https://capsid.vbcf.ac.at/events/
ANNOTATION and SUBMISSION DATA OF THE LECTURE (pdf)
Funding programme of the CAPSID project: Interreg V-A Slovakia-Austria
Organizers:
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