Seminar Speaker Series – Dr. Jana Neuhold (29. 04. 2021)

Lecture by Dr. Jana Neuhold from VBCF, AT entitled „GoldenBac: A simple, highly efficient, and widely applicable system for construction of multi-gene expression“ from the CAPSID Seminar Speaker Series, will take place on 29 April 2021 at 2 p.m.via online broadcasting via the ZOOM application.

ANNOTATION:

Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control of its own promoter and terminator. Current methods are based either on serial rounds of combination of several vectors containing loxP sites via the Cre-lox technology, or on multiple rounds of gene combination via PCR or other methods. These methods are multi-step, have lower efficiencies than single gene cloning, and may require laborious processes to verify that all genes of interest are present in the final product. Here, we describe a rapid and simple Golden Gate-based system for the generation of multi-gene expression constructs compatible with baculovirus expression vector systems (BEVS) using either Tn7 transposition or KO1629-based homologous recombination, which we refer to as “GoldenBac”.

More information about lecture and registration on the project web site: https://capsid.vbcf.ac.at/events/ 

ANNOTATION and SUBMISSION DATA OF THE LECTURE (pdf)

Funding programme of the CAPSID project: Interreg V-A Slovakia-Austria

 Organizers: